successful cryopreservation of in vitro derived bovine blastocysts in microcapillary pipette tips

Successful cryopreservation of in vitro derived bovine blastocysts in microcapillary pipette tips

The open pulled straw has been used effectively to vitrify preimplantation embryos because of geometricfeatures that allow rapid rates of temperature exchange. One possible inexpensive alternative to the openpulled straw are commercially-available microcapillary pipette tips commonly used for electrophoresis. Themain purpose of this study was to compare the survival rates of in vitro produced b...

The Effect of Pestivirus on the in vitro Development of Bovine Morula and Blastocysts.

Effect of Culture System on Developmental Competence, Cryosurviva and DNA-Fragmentation of In Vitro Bovine Blastocysts

P-83: Cloned Sheep Blastocysts Derived from Oocytes Enucleated Manually Using A Pulled Pasteur Pipette

Background: The potential applications of a simplified method of somatic cell nuclear transfer (SCNT) that is improved in both efficiency and throughput is considerable. Technically, a major step of SCNT is to produce large pools of enucleated oocytes (cytoplasts) efficiently, a process that requires considerable micromanipulation skill and expensive equipment. Here, we have devel oped an efficient...

Embryonic Stem-like Cells Derived from in vitro Produced Bovine Blastocysts

The aim of this work was to study the derivation of bovine embryonic stem-like (ES-like) cells from the inner cell mass (ICM) of in vitro produced blastocysts. The ICMs were mechanically isolated and six out of seventeen (35%) ICMs could attach to a monolayer of murine embryonic fibroblasts (MEF).Ten days after, primary outgrowths were mechanically dissected into several small clumps and transf...

Effect of the bovine oviductal fluid on in vitro fertilization, development and gene expression of in vitro-produced bovine blastocysts.

Oviductal microenvironment generally provides better conditions for early embryo development than the conventional in vitro system. In an attempt to simulate the oviduct conditions or the main potentially influencing factors, the effect was studied of a bovine oviductal fluid (bOF) treatment applied prior to IVF on (i) IVF parameters, (ii) cleavage rate, (iii) blastocyst yield and (iv) blastocy...